Denver remains the only Colorado municipality on the U.S. Department of Justice’s updated list of “sanctuary jurisdictions.”

Released on Tuesday morning, the updated list identified 22 cities and counties around the country, a significant decrease from the more than 500 named in the initial DOJ report published at the end of May.

The update comes as Denver, along with nearly 50 other jurisdictions, is suing the Trump administration over its threats to withhold federal funding. Earlier, the Trump administration sued Denver and Colorado, asking a judge to overturn state and city policies restricting local cooperation with federal immigration officials.

Denver sues Trump administration, again, over threats to withhold federal funding

“Even as President Trump continues to willfully ignore the rule of law by bullying cities into doing his dirty work, Denver will always stay true to our values,” Denver Mayor Mike Johnston said in a statement last month, when the city filed its lawsuit over federal funding. “Time and time again, Denver has shown that we enforce the law without fear or favor and work with federal authorities to bring criminals to justice.”

Johnston and Denver’s policies have been at odds with Trump and his administration’s immigration priorities since the president assumed office in January. Johnston also made headlines for saying he would be willing to go to jail defending his city’s immigration stance, a statement that invited both praise and criticism.

In an April executive order, President Donald Trump said “sanctuary” jurisdictions have obstructed the enforcement of immigration laws.

In the same order, Trump called the actions of some state and local officials a “lawless insurrection against the supremacy of federal law and the federal government’s obligation to defend the territorial sovereignty of the United States.”

Alongside Denver, the other cities on the updated DOJ list included New York City, Los Angeles, Boston, Chicago and San Francisco.

Broadly speaking, a “sanctuary jurisdiction” refers to a community that expressly refuses to or forbids cooperation with federal authorities on enforcing immigration laws. In 2017, the Denver City Council passed an ordinance that prohibits “city employees from collecting information on immigration or citizenship status; prohibits the sharing of any other information about individuals for purposes of immigration enforcement; and, memorializes predominant practices by prohibiting use of city resources or City cooperation with civil immigration enforcement.”

At the state level, a law, which lawmakers expanded this year, expressly prohibits all state agencies and political subdivisions from sharing or inquiring into individuals’ personal identifying information for the purposes of enforcing immigration laws. The law imposes a civil fine of $50,000 for each violation.

The Trump administration is threatening further lawsuits against the jurisdictions.

“Sanctuary policies impede law enforcement and put American citizens at risk by design,” Attorney General Pam Bondi said in a statement. “The Department of Justice will continue bringing litigation against sanctuary jurisdictions and work closely with the Department of Homeland Security to eradicate these harmful policies around the country.”

Colorado is also one of 13 states on the list; others included California, Oregon, New York and Washington. Only four counties appeared on the updated list, none of which is in Colorado.

The initial May report listed 41 of Colorado’s 64 counties as having sanctuary policies. It also named 14 cities, including Denver, Fort Collins, Lakewood and Vail.

Jurisdictions, such as El Paso County and Aurora, were originally on the initial list before they were quickly removed.

“We were mistakenly placed on the list and then correctly taken off,” Aurora Mayor Mike Coffman said at the time. “The city of Aurora has never been a sanctuary city. Unlike Denver, we have always cooperated with federal immigration authorities to the fullest extent allowed under state and federal law.”

Leaders from other jurisdictions around the country also said their municipality’s placement on the initial list had been a mistake.

Baltimore Mayor Brandon Scott denounced his city’s placement on the initial list on social media, saying it does not have jurisdiction over its jails, a key area of cooperation between local law enforcement and ICE.

The city of Las Vegas also issued a statement on social media, saying it hoped to “clear up this misunderstanding” about its inclusion.

The Washington Examiner contributed to this report.